Oligo concentration calculator. Example. 55 OD (312. Scenario: 50 pmol oPools Oligo Pool Quantity: 50 pmol oPools Oligo Pool / 1000 = 0. Enter sequence and parameters to calculate Jan 18, 2013 · Figure 3. Our calculator is a convenient tool of its kind that takes into consideration the amount of oligos, the desired concentration, and the volume of the Use our oligo calculators to determine volumes needed to resuspend your DNA oligos to desired concentrations, estimate the percentage of full-length product for different oligo lengths, and calculate final yield based on oligo length, synthesis scale, and purification method. Standardization of reagents for comparative studies: To compare experimental results reliably, it’s crucial to standardize reagents like oligonucleotides across different experiments. 06. 6log (required salt concentration / 0. Confirm the desired concentration by measuring the A 260 of the solution and calculate the concentration using the extinction coefficient (ε) provided on the oligo spec sheet (A = εCL). Oligonucleotide 1: delivered with 10. Here’s what each part means: C – This is how much of the oligonucleotide we have. 98) and the addition of two hydrogens (2. Oligo Calculation Tool. The relationship between the free energy and the concentration of reactants and products at equilibrium is given by. Calculate the volumes of the oligonucleotides needed based on their concentrations and the total volume of the annealing reaction. Free Mg ++ Ion Concentration The peptide content is the amount of pure peptide (net weight) in a product and a lot specific value, because it depends on the purity and assay (content) of the lot. and solving for temperature T gives. Amino Acid Proportions Oct 21, 2013 · Let a free copy number calculator do it for you! A convenient, free-access copy number calculator to convert nanograms to copy number for real time PCR (RT-PCR) was developed by former IDT customer, Andrew Staroscik, who has since left the bench. Enter or Paste Sequence: Input the oligonucleotide sequence in the text area provided. Additional features include sgRNA Template Oligo Design and qPCR library quantification. c = concentration in mol L-1. Easily create a stock solution by allowing the resuspension calculator take the guesswork out of dissolving your oligo. OD of 1 is equal to nanoMolar. 05 g/mol), if we assume the DNA absorbance to be 4,900, no dilution, and standard cuvette size, we can calculate the concentration. Calculation for oligo quantity: Calculation for oligo concentration: Oct 3, 2023 · Synthesising your own oligos provides a secure supply chain, faster turnaround times and cost control. Sequence Information. OligoCalc has a familiar ‘calculator The melting temperature Tm of a DNA double strand is defined as the temperature at which 50% of the double strand have been denatured, thus being present in single stranded form. Compare different purification methods and order custom DNA oligos online. The desired concentration of the duplex oligonucleotide is 50 µM. ng/µl. The Dotmatics digital science platform provides the first true end-to-end solution for scientific R&D, combining an enterprise data platform with the most widely used applications for data analysis, biologics, flow cytometry, chemicals innovation, and more. Reverse Complement Strand (5' to 3') is: 5' modification (if any) 3' modification (if any) Select molecule. ; ε260 – This is a special number that tells us how good the oligonucleotide is at absorbing light. 41°(%GC) - 675°/length of oligo . Options average mass monoisotopic mass Oligo Concentration: The default value = 250. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity. The T m value reported on our spec sheets uses a 25 µM oligonucleotide and a 50 mM salt concentration. we recommend using a ssDNA oligo as Our oligonucleotide tools and calculators are designed to enhance the efficiency, accuracy, and outcomes of your molecular biology research. 8 Use our oligo calculators to determine volumes needed to resuspend your DNA oligos to desired concentrations, estimate the percentage of full-length product for different oligo lengths, and calculate final yield based on oligo length, synthesis scale, and purification method. Oligo Concentration Calculator. Try our free oligonucleotide calculator. The copy number calculator can be found here. 195M using the OligoCalculator. Free Mg ++ Ion Concentration Sep 21, 2012 · Resuspension Calculator. Automatically calculate the length, melting temperature, %GC content, molecular weight, and nanoMolar equivalent for your oligo sequence. The PrimerQuest™ Tool Even though you have access to IDT’s PrimeTime Predesigned qPCR Sequence Database, that contains predesigned qPCR sequences for human, mouse, and rat targets, we If your desired final concentration is µM per oligo, use this equation to determine your quantity: pmol per oligo / 1000 = # nmol per oligo Example 1. Calculate the amount of oligo (nmol) using the formula: Amount of oligo (nmol) = A260 x nmol per OD x dilution factor. The default value = 100. T m varies with oligonucleotide concentration. C is the desired stock concentration. The NanoDrop software will do this for you, when you choose either "custom" or "oligo" option from the sample type menu. (1998) PNAS, 95:1460-5) Tm is adjusted for MgCl2 and DMSO concentration based on von Ahsen et al. Oligo Properties Calculator. It does not take into account dNTP or Mg 2+ concentrations. Conversely, you can calculate the molarity of a nucleic acid solution prepared by dissolving a certain amount of it in a specified volume of a solvent. 05 nmol Final concentration: 1 µM Resuspension calculator (Figure 1): Pooling Calculator. This is used to convert the weight (weight concentration) into the molar quantity (molar concentration) for nucleic acids, and vice versa. > For example, if you paste 10 lines, the calculator will assume 10 sequences. 0 mM. Step 2: click on the Analyze button. 5° + 0. DNA concentration: nM (default=50) Salt Eurofins Genomics' Oligo Analysis Tool is a multifunctional tool, which gives you the option of checking your oligos before you order them. The percentage of full-length oligonucleotide depends on the coupling efficiency of the chemical synthesis. An open portfolio of interoperable, industry leading products. How it works 1. The data is listed in Table 2. Dilute solution to a desired concentration (mass/vol). Protein Properties. The sequence can be typed into the software and the sequence-specific extinction coefficient and concentration factor will be automatically calculated. This calculator is useful for diluting primers and DNA oligos. Use the OligoAnalyzer Tool to calculate your coefficient of extinction and oligo concentration. acid [kb]: Nucleic Acid Calculator - Software Toolbox for Oligo Chemists. • Both instruments were able to determine the oligonucleotide concentration without dilution for the 3 mg/mL sample at 260 nm and 295 nm. Suppose you have 5 nmol of dry oligo and you want to prepare a 100 µM stock solution: Delivered Amount of Dry Oligo: 5 nmol The subtraction of 61. To get an accurate T m for your specific application, all of these concentrations should be entered and adjusted to match the reaction conditions you plan to use. This tool can be used to determine the concentration and amount of an oligo based on its optical density (OD), extension coefficient and volume. Mix—add the 2 oligo strands together in equal molar amounts. Use an absorption measurement from a spectrophotometer and the extinction coefficient to quickly confirm the concentration of oligos Enter Oligonucleotide Sequence: Parameter sets: Input style: DNA Therapeutic Easily create a stock solution by allowing the resuspension calculator take the guesswork out of dissolving your oligo. Free Mg ++ Ion Concentration Oligo Concentration: The default value = 250. Free Mg ++ Ion Concentration Need an oligo calculator? IDT's free, online OligoAnalyzer™ Tool will analyze an input sequence and provide its molecular weight (MW) as well as GC content, melting temperature, µg/OD, nmol/OD, and extinction coefficient. 8 Oligo Concentration: The default value = 250. Ligation Calculator. This tool will calculate the amount of stock solution needed to achieve a desired concentration in a given volume. Each oligo has its own Ɛ, and this value changes at each wavelength. You can provide the input as nmol, mg, or as OD 260 with MW or extinction coefficient (provided on the spec sheet sent with the oligo). This eliminates the need for an online calculator to determine the proper extinction coefficient. Enter Oligonucleotide Sequence Below. Free Mg ++ Ion Concentration Use the oligo dilution calculator to determine the dilution of oligo solutions and create aliquots for working stocks. Required Tm= Known Tm + 16. 2. When measuring nucleic acid samples with UV-Vis Molarity Calculator. The first equation shown in Figure 6 demonstrates the mathematical relationship between molarity Sep 5, 2023 · The Dilution Calculator takes an oligo stock solution of higher concentration and determines how much volume to dilute down to final working concentration. g. Length of the oligo, base composition and concentration of an oligonucleotide as well as the salt concentration in the solution have a key influences on the Tm value. nM. Thermo Fisher offers simple examples and tips to help you calculate primer and probe concentrations. 9 Oligo Calculator. 1. Additionally, it allows for customisable synthesis and enhanced quality control, ensuring the accuracy and purity of your oligonucleotides. Predict oligo secondary structure or possible duplexes with HAIRPIN, SELF-DIMER, and HETERO-DIMER options. Use this tool for your scientific calculations and conversions for DNA and RNA. Where: T m = melting temperature in °C ΔH = enthalpy change in kcal mol-1 (accounts for the energy change during annealing / melting) A = constant of -0. K + encourages annealing by reducing charge repulsion between the negatively charged primers and template backbones. The code will automatically be placed onto the appropriate position of the sequence, but you also can move and rearrange any internal modification codes you select. Step 3: read out your results. Final Concentration: Enter the specifications for your oligo modifications To add a modification code, select the Add Mod button to the left of its description. The first equation shown in Figure 6 demonstrates the mathematical relationship between molarity Oligo Concentration: The default value = 250. Use our oligo calculators to determine volumes needed to resuspend your DNA oligos to desired concentrations, estimate the percentage of full-length product for different oligo lengths, and calculate final yield based on oligo length, synthesis scale, and purification method. Final concentration of 1 µM per oligo. dsDNA ssDNA RNA olig Size of the nucl. Oct 21, 2013 · T m and oligonucleotide concentration. Enter your primer or other oligo sequence; Adjust calculation options if desired; Choose a function: Select ANALYZE for easy, one-click access to a T m calculator, GC content calculator, extinction coefficient calculator, and more. The Resuspension Calculator (Figure 2) determines the volume needed to resuspend a dry-shipped oligo for a desired final concentration. Extinction Coefficient Calculation - The extinction coefficient is calculated with the following method: ε260 = [(Sum of ε260 for all bases*) + (ε260 for all modifications*)] x 0. Select Type: Choose the type of oligonucleotide (ssDNA, dsDNA, ssRNA). Primer Concentration (nM) Step 3. Jun 15, 2012 · Heating (up to 94°C) and vortexing will facilitate resuspension. By using our site you acknowledge that you have read and understand our privacy notice . Example Calculation. The concentration must be determined for each oligonucleotide as outlined above using either the longform or shortcut calculation. Calculate basic physical and chemical parameters of a nucleic acid molecule. K +, present in the reaction mixture. The volume of diluent needed (V) is calculated by dividing the amount of dry oligo (m) by the desired stock concentration (C). 05 nmol Final concentration: 1 µM Resuspension calculator (Figure 1): Oligo Concentration: The default value = 250. Please input the oligo sequence in the text-box below. Key to the Beer-Lambert law is the extinction coefficient, which measures how strongly the absorbing bases reduce the intensity of the UV light from incidence to transmission. Explore a range of oligonucleotide designs, including antisense oligos, siRNA, gapmers, and mixmers. disorder) R = gas constant of 0. See more SciTools™ Web Tools from IDT. OD calculations are for single-stranded DNA or RNA. Likewise, you can specify the units of final The concentration of oligonucleotide in your solution can then be calculated by simply entering the measured A260 in the concentration calculator box. Base Dye Ratio Calculator. 1 Stock Concentration (uM or pmol uL-1): ¶ T m calculation for DNA oligo is based on the following equation: T m = 81. Mar 31, 2017 · Calculating concentration Oligo concentration describes the amount of oligos present in a specified volume, such as one liter or microliter. If you would like an accurate MW for restriction enzyme cut DNA, please use: OligoCalc is a web-accessible, client-based computational engine for reporting DNA and RNA single-stranded and double-stranded properties, including molecular weight, solution concentration, melting temperature, intra-molecular hairpin loop formation, etc. Use an absorption measurement from a spectrophotometer and the extinction coefficient to quickly confirm the concentration of oligos Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. Measure the absorbance of this dilution at 260 nm (A 260). Resuspension calculations can be made using the yield information that is provided both on the oligo tubes themselves and on the IDT oligo product Apr 22, 2007 · We developed OligoCalc as a web-accessible, client-based computational engine for reporting DNA and RNA single-stranded and double-stranded properties, including molecular weight, solution concentration, melting temperature, estimated absorbance coefficients, inter-molecular self-complementarity estimation and intra-molecular hairpin loop formation. Three easy steps for determining oligonucleotide extinction coefficient and concentration. Calculate molecular weights for nucleic acids like DNA, RNA, and modified backbones including Phosphodiester (PO) and Phosphorothioate (PS). Enter Oligo Sequence in Box: Length : Melting Temperature (Tm) °C %GC content Molecular Weight: daltons (g/M) OD of 1 is equal to picoMolar. ss oligo concentration (ug/ml) = OD 260 x MW x 1000 / ε 260 where MW = molecular weight of oligo (non-phosphorylated) and ε 260 = extinction coefficient of oligo at 260nm A path length of 1 cm is assumed for the absorbance. Use an absorption measurement from a spectrophotometer and the extinction coefficient to quickly confirm the concentration of oligos in solution. Step 1: paste or type in the oligo sequence of interest. 195M), where the Known Tm is calculated from 0. Tm calculator using the Nearest-Neighbor method (NN paramters are based on SantaLucia J Jr. Please select the oligo type (DNA or RNA) to be analysed 2. Nucleotide base codes. Library Plexity Unit of Measure for Library. Enter a name for the the oligo (use _, -, and alphanumeric characters) 3. The average efficiency is close to 99%. Verify the DNA concentration by preparing duplicate dilutions and determining the A260. Since we have already calculated the molecular weight (1503. DNA calculations to convert µg to pmol for double-stranded and single-stranded DNA, convert micrograms of DNA to pmol ends, calculate vector:insert molar ratio and convert OD260 readings to µg/ml. JaMBW Chapter 3. Also calculate molarity of solutions, perform molar conversions, calculate dilutions and perform other calculations common in molecular biology labs. Start optimizing Oligo Tm Calculator. These numbers are used to calculate the analysis constant needed for Nanodrop concentration calculations. The peptide calculator uses the analytical data which are documented in the respective Analytical data sheet (ADS) for Bachem’s products as shown in this example. According to the formula concentration of desoxynucleotide triphosphate [dNTP] must be smaller than concentration of divalent cations. Use an absorption measurement from a spectrophotometer and the extinction coefficient to quickly confirm the concentration of oligos Use our oligo calculators to determine volumes needed to resuspend your DNA oligos to desired concentrations, estimate the percentage of full-length product for different oligo lengths, and calculate final yield based on oligo length, synthesis scale, and purification method. version {{appVersion}} Oligonucleotides purchased through LGC Biosearch Technologies arrive with data sheets containing the extinction coefficient and molecular weight of each oligonucleotide. Jun 18, 2024 · m is the amount of dry oligo. How to calculate the oligo concentration from absorbance at 260 nm? To quantify your Oligonucleotides, make an aliquot of the resuspended Oligonucleotides to a final volume of 1 ml of dH 2 O and vortex for a few seconds. Choose a DNA, RNA, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. Free Mg ++ Ion Concentration Enter your primer or other oligo sequence; Adjust calculation options if desired; Choose a function: Select ANALYZE for easy, one-click access to a T m calculator, GC content calculator, extinction coefficient calculator, and more. Similarly to different oligonucleotide calculators that can be found on the internet, our Oligo calculator will provide the molecular weight of the desired oligonucleotide with or without a DMT group and 2’-O modifications. Jun 18, 2024 · Optical Density to Nanomolar (OD): The conversion of optical density to nanomolar concentration for ssDNA. Tm Calculation for Oligos: Combined concentration of K + and Na + in the reaction: mM: Primer concentration in the reaction: nM: The oligo has a 5' -phosphate group Enter your primer or other oligo sequence; Adjust calculation options if desired; Choose a function: Select ANALYZE for easy, one-click access to a T m calculator, GC content calculator, extinction coefficient calculator, and more. Oligo Calculator. version 1. Jan 18, 2013 · Figure 3. 0 nM. Mongo Oligo Mass Calculator v2. Jan 14, 2014 · IDT offers a Resuspension Calculator as part of our SciTools™ suite, which is useful for calculating the volume of buffer to add to a dried down oligo to obtain a desired concentration. e. Substituting the two equations gives us. Free Mg ++ Ion Concentration Use our oligo calculators to determine volumes needed to resuspend your DNA oligos to desired concentrations, estimate the percentage of full-length product for different oligo lengths, and calculate final yield based on oligo length, synthesis scale, and purification method. This tool calculates melting temperature (Tm), molecular weight (MW), Extinction Coefficient (OD/μmol, μg/OD) for oligos. . To use the Oligo Analysis tool 1. Used to determine how […] The common Tm formulas for calculating the theoretical Tm of an oligo are: [OD260 DNA concentration determination calculator ] [Yeast Two-Hybrid] Loading Library Concentration Conversion Calculator Library Concentration Conversion Calculator Consider the xGen™ Normalase™ Module and xGen Normalase indexing primers for enzymatic normalization of up to 1,536 libraries. Please note: this calculation works well for synthesized oligonucleotides. Applications of the Oligo Dilution Calculator Sep 21, 2012 · Resuspension Calculator. Step 2. OligoCalc algorithm recognizes 5’ and 3’ chemical modifications and predicts potential hairpin loops. Calculate the mass or volume required to prepare a nucleic acid solution of specified molar concentration. Molarity Calculator. The concentration of dNTPs is included to the formula beacause of some magnesium is bound by the dNTP. This multi-functional tool facilitates the theoretical calculation of several oligo sequence dependent properties. OligoCalc is a tool to calculate the melting temperature, concentration, molecular weight, absorbance at 260 nanometers, and physical properties of single- and double-stranded DNA and RNA molecules. Likewise, you can specify the units of final This calculator simplifies the conversion of a dry oligo into a usable stock solution at the desired concentration, essential for accurate experimental setups. Dilute solution to a desired Molarity. 02). The handy and simple interface of this calculator makes it a convenient tool to use on daily basis for oligo calculation. Free Mg ++ Ion Concentration The concentration of each oligonucleotide needs to be 2X the desired concentration of the duplex oligonucleotide. 9 nmol); verify measured OD amount with spectrophotometer. OligoCalc has a familiar x91calculatorx92 look and feel, making it readily understandable and usable. This step is critical to If your desired final concentration is µM per oligo, use this equation to determine your quantity: pmol per oligo / 1000 = # nmol per oligo Example 1. 96 gm/mole from the oligonucleotide molecular weight takes into account the removal of HPO 2 (63. This tool will calculate the mass needed to generate a solution based on the desired molarity, MW, and volume, or the molarity based on MW, volume, and mass. 05 nmol Final concentration: 1 µM Resuspension calculator (Figure 1): Choose a DNA, RNA, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. Unlock the potential of bioconjugates for efficient uptake and delivery. Calculate Properties. Sequence (Paste the raw sequence, not fasta format): Required volume (V1) = desired final volume (V2) × desired concentration (M2) / stock concentration (M1) M1 × V1 = M2 × V2 represents the principle of conservation of mass in the context of dilution, ensuring the moles of solute before and after dilution remain constant. Select Source: Choose the source (PCR or Restriction Cut). Use the formula below to calculate the concentration of Jan 29, 2018 · Thus, for quantification, IDT recommends that you determine and apply an oligo-specific conversion factor. Bio Synthesis Provides Oligo Batch Calculator. The concentration of oligonucleotide in your solution is then calculated by: Oligo Conc = Absorbance Factor x A 260. for Oligonucleotide Concentration The equivalency assessment focused on the use of 3mg/mL sample concentration and followed the strategy outlined in Figure 2. 99n-1 Therefore, 79% of the oligonucleotide molecules in the tube are 25 bases long; the rest are <25 bases. Sep 28, 2007 · OligoCalc provides a convenient web interface for calculating the physical properties of DNA and RNA oligonucleotides including melting temperature, molecular weight, %GC content and absorbance coefficient for a given oligonucleotide sequence. 𝜀 260 = extinction coefficient of the oligonucleotide at 260 nm in L ⋅ mol-1 ⋅ cm-1. You can calculate: Molecular Weight (DMT-ON/OFF), Molar Extinction Coefficient, Concentration, Theoretical Melting Temperature, GC Content, Inverse Complement Important notes The OligoSpec calculator outputs the physical properties for a particular oligo design. User Instructions. Sequence (5'-->3') Memories Calculation. 8 If your desired final concentration is µM per oligo, use this equation to determine your quantity: pmol per oligo / 1000 = # nmol per oligo Example 1. 9, to adjust for hyperchromicity. 00199 kcal K-1 ᐧ mol-1 (constant Verify the DNA concentration by preparing duplicate dilutions and determining the A260. Calculate Oligonucleotide Properties This tool allows You to calculate the physical properties of modified and mixmer oligonucleotides including commonly used 3' and 5' modifications. 15. These tools enable the precise quantification, dilution, and resuspension of your research oligos, minimizing experimental variability and maximizing reproducibility. 0108 kcal K-1 ᐧ mol-1 (accounts for helix initiation during annealing / melting) ΔS = entropy change in kcal K-1 ᐧ mol-1 (accounts for energy unable to do work, i. Attained concentration of monovalent cations is used to calculate oligo/primer melting temperature. Oligo concentration alone can cause T m to vary by ±10°C . Free Mg ++ Ion Concentration Dilution Calculator. Oligo Concentration: The default value = 250. Use the MicroArray module of the NanoDrop software for measuring oligonucleotides with modifications. Find basic concepts and formulas for calculating concentration of solution, primer pcr concentration or dilution, and reconstitute/recover lyophilized powder. Clinical Chemistry 47: 1956-61 (2001) Enter your primer or other oligo sequence; Adjust calculation options if desired; Choose a function: Select ANALYZE for easy, one-click access to a T m calculator, GC content calculator, extinction coefficient calculator, and more. ℓ = pathlength in cm. It also facilitates the set up of experiments by calculating the adequate amounts and dilutions for your oligo solutions. Dec 30, 2015 · OligoCalc: Oligonucleotide Properties Calculator. While folding of a single oligonucleotide is concentration independent, the T m is strongly influenced by oligo concentration when 2 or more nucleic acid strands interact. Library Size Library Concentration (ng/µl) Library Concentration (nM) Library Volume Nov 20, 2019 · A problem with using OD to measure concentration is that the way each substance scatters light is unique to that substance. 6 µg, 49. This is commonly conveyed as micromolarity (µM), or the number of micromoles (1 x 10-6 moles) per liter. NEBioCalculator. Jul 20, 2024 · An oligo resuspension calculator is a tool used to determine the volume of the diluent needed to dissolve a given amount of oligonucleotides (short DNA or RNA molecules) to a desired concentration. Nov 20, 2019 · A problem with using OD to measure concentration is that the way each substance scatters light is unique to that substance. Select the type of oligo—DNA or RNA—to be analyzed 2. Reagent Dilution Calculator. NEBuilder Protocol Calculator can generate a protocol for your NEBuilder HiFi reaction when given a set of DNA fragments. The unique properties of each substance are accounted for using a variable called the extinction coefficient (Ɛ), which you use to calculate concentration. The first equation shown in Figure 6 demonstrates the mathematical relationship between molarity Choose a DNA, RNA, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. We can assume that the concentration of DNA and the concentration of the DNA-primer complex are equal, so this simplifies the equation considerably. Enter a sequence in Oligo concentration (µM) DNA or RNA. Dilution Calculator The relationship between the free energy and the concentration of reactants and products at equilibrium is given by Substituting the two equations gives us and solving for temperature T gives We can assume that the concentration of DNA and the concentration of the DNA-primer complex are equal, so this simplifies the equation considerably. Prepare oligo working stocks with confidence and precision. Inputting these values into the DNA concentration calculator gives the final result of 149. Use the formula below to calculate the Analysis Constant (AC): Oligo Concentration: The default value = 250. Oligo DNA or Oligo RNA applications. 1. Use these calculators to determine oligo resuspension, full-length product, and yield based on oligo length, concentration, and synthesis scale. Monovalent Ion Concentration: This is the concentration of all monovalent ions, e. 39 mg/mL. Free Mg ++ Ion Concentration Oligo quantity (nmol) is directly proportional to the oligo concentration (µM) and volume (µL). RUO23-1753_001. Calculate Tm (melting temperature Note: When entering decimal values in concentration fields, Enter Oligo Sequence. Free Mg ++ Ion Concentration Oligo calculator Calculate the Tm, dimere formation and stem /loop structures for an oligonucleotide sequence. This calculator is useful for diluting DNA samples. ; A260 – This is how much light the oligonucleotide absorbs at 260 nm. Either the longform or shortcut calculation can be used to verify quantification values provided with technical datasheets. Where: A 260 = absorbance at 260 nm. To calculate the percentage of full-length oligonucleotide, use the formula: 0. uqyfbqv ycvezb drpu ggklw pwmxf vylsof mwsqa uttr smqp jfaa .